Properties of the platelet retention (von Willebrand) factor and its similarity to the antihemophilic factor (AHF).
نویسندگان
چکیده
منابع مشابه
Quantitative assay of a plasma factor deficient in von Willebrand's disease that is necessary for platelet aggregation. Relationship to factor VIII procoagulant activity and antigen content.
In a previous paper, we showed that the abnormality of ristocetin-induced platelet aggregation in platelet-rich plasma in 10 patients with von Willebrand's disease could be corrected by a factor in normal plasma that was present in the same fractions as factor VIII procoagulant activity (antihemophilic factor, AHF, VIII(AHF)) when prepared by chromatography on Bio-Gel 5 M (Bio-Rad Laboratories,...
متن کاملThe relationship of the properties of antihemophilic factor (factor VIII) that support ristocetin-induced platelet agglutination (factor VIIIR:RC) and platelet retention by glass beads as demonstrated by a monoclonal antibody.
A monoclonal antibody to human antihemophilic factor (AHF, factor VIII) was derived from BALB/c mouse spleen cells fused with P3x63Ag8 mouse plasmacytoma cells. This antibody, harvested from culture medium or ascites fluid, reacted with purified AHF and with plasmas with normal subjects or classic hemophiliacs, as measured by enzyme-linked immunosorbent assay (ELISA), but not with plasmas from ...
متن کاملThe Relationship of the Properties of Antihemophilic Factor ( Factor VIII ) That Support Ristocetin - Induced Platelet Agglutination
A monoclonal antibody to human antihemophilic factor (AHF. factor VIII) was derived from BALB/c mouse spleen cells fused with P3x63Ag8 mouse plasmacytoma cells. This antibody. harvested from culture medium or ascites fluid. reacted with purified AHF and with plasmas from normal subjects or classic hemophiliacs, as measured by enzymelinked immuonsorbant assay (ELISA). but not with plasmas from p...
متن کاملDefective ristocetin-induced platelet aggregation in von Willebrand's disease and its correction by factor VIII.
The antibiotic ristocetin, in concentrations of 1.0-1.5 mg/ml, aggregated normal platelets in citrated platelet-rich plasma by a mechanism in which the release reaction played only a minor role. Platelet aggregation by ristocetin in a concentration of 1.2 mg/ml was absent or markedly decreased in 10 patients with von Willebrand's disease. Lesser degrees of abnormality were obtained with a conce...
متن کاملMacromolecular factor VIII complex: functional and structural heterogeneity observed in von Willebrand swine with transfusion.
The physiologic activities concerned with hemostasis and associated with the Factor VIII macromolecular complex were investigated in swine with von Willebrand's disease after infusion of cryoprecipitate, a lyophilized Factor VIII concentrate, or porcine serum. Immediately after each infusion the various activities antihemophilic factor, von Willebrand platelet aggregating factor, and Factor VII...
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ورودعنوان ژورنال:
- Blood
دوره 41 6 شماره
صفحات -
تاریخ انتشار 1973